Western Blot: myosin X Antibody [22430002] - Detection of Myo10 in HeLa cells at a dilution of 1:800 with 1 mg/mL stock. The predicted molecular weight of MYO10, based on the amino acid sequence, is 237 kD. MYO10 appeared at approximately 250 kD on this gel.
Immunocytochemistry/Immunofluorescence: myosin X Antibody [22430002] - staining of Myo10 at a dilution of 1:800 with 1.0mg/mL stock overlapped with immunofluorescence staining of Actin at a dilution of 1:800. Image courtesy of MM DiVito and RE Cheney, Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill.
Immunohistochemistry: myosin X Antibody [22430002] - Most of the normal tissues showed moderate cytoplasmic positivity. Strong staining was observed in stomach, rectum, exocrine pancreas, urothelium and in placenta. Hepatocytes were negative. Malignant tissues exhibited similar staining properties as normal cells. Occasional tumor cases displayed strong cytoplasmic immunoreactivity. Most cases of malignant lymphoma, testicular and prostate cancers were negative or weakly stained. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunocytochemistry/Immunofluorescence: myosin X Antibody [22430002] - staining of Actin at a dilution of 1:800. Image courtesy of MM DiVito and RE Cheney, Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill.
Immunocytochemistry/Immunofluorescence: myosin X Antibody [22430002] - Myosins are actin-based motor molecules with ATPase activity. MYO10 plays a role in regions of dynamic actin and is presumed to serve in intracellular movements. Immunofluorescence staining of Myo10 at a dilution of 1:800 with 1.0 mg/mL stock. Image courtesy of MM DiVito and RE Cheney, Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill.
ELISA: myosin X Antibody [22430002]