CaM Kinase II alpha Antibody (6G9)

• Supplier: Novus Biologicals
• Catalog: NB100-1983
• Prices: $169.00, $349.00
• Sizes: 25 µg, 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 6G9
• Applications: WB ELISA ICC/IF IHC IHC-P IP RIA
• Species Reactivities: Rat
• Antigen: Detects approx. 50-60kDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.Partially purified rat CaMKII
• Purity/Format: Protein G purified
• Description: Mouse Monoclonal
• Gene: Camk2a
• Conjugate: Unconjugated

Product images

Western Blot: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.

Immunohistochemistry: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4 degrees C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.

Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody (6G9) [NB100-1983] - Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.