MHC Class II Antibody (M5/114.15.2)

• Supplier: Novus Biologicals
• Catalog: NBP1-43312
• Prices: $209.00, $259.00, $329.00
• Sizes: 50 µg, 100 µg, 500 µg
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone: M5/114.15.2
• Applications: WB FC IHC IHC-F IHC-P IP
• Antigen: The immunogen for this antibody was MHC Class II.
• Purity/Format: Protein A or G purified
• Description: Rat Monoclonal
• Gene: HLA-DMA
• Conjugate: Unconjugated

Product images

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the PE conjugate of NBP1-43312. Analysis of mouse splenocyte suspensions using MHC Class II PE and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Staining of C57BL/6 splenocytes with 0.06 ug of Rat IgG2b K Isotype Control Purified (cat. NBP1-43323) (open histogram) or 0.06 ug of Anti-Mouse MHC Class II (I-A/I-E) Purified (filled histogram) followed by Anti-Rat IgG FITC (cat. NBP1-69751). Total viable cells were used for analysis.

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Allophycocyanin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC and 0.015 micrograms of Rat IgG2b ? Isotype Control APC (left) or 0.015 micrograms of Anti-Mouse MHC Class II (I-A/I-E) APC (right).

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Biotin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with 0.015 ug of Rat IgG2b K Isotype Control Biotin (open histogram) or 0.015 ug of Anti-Mouse MHC Class II (I-A/I-E) Biotin followed by Streptavidin PE (filled histogram).

Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the FITC conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e and 0.06 micrograms of Rat IgG2b k Isotype Control FITC (left) or 0.06 micrograms of Anti-Mouse MHC Class II (I-A/I-E) FITC (right). Cells in the lymphocyte gate were used for analysis.