Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD302/CLEC13A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6367) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD302/CLEC13A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human peripheral blood monocytes were stained with Sheep Anti-Human CD302/CLEC13A Affinity Purified Polyclonal Antibody (Catalog # AF6367, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).