Western Blot: FAK Antibody [AF4528] - Lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 uM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 ug/mL of Human Phospho-FAK (Y397) Antigen Affinity-purified Polyclonal Antibody (R&D Systems catalog # AF4528), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (R&D Systems catalog # HAF008). A specific band was detected for Phospho-FAK (Y397) at approximately 122 kDa (as indicated). This experiment was conducted under reducing conditions.
Simple Western: FAK [p tyr397] Antibody [AF4528] - Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-FAK (Y397) at approximately 123 kDa (as indicated) using 10 ug/mL of Rabbit Anti-Human Phospho-FAK (Y397) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4528). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.