Stat3α (D1A5) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 8768
• Prices: $109.00, $277.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D1A5
• Applications: WB IP IHC-P ICC/IF ChIP
• Species Reactivities: Human, Mouse, Rat, Hamster, Monkey, Pig
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Stat3α protein.
• Description: Rabbit Monoclonal
• Gene: STAT3

Product images

Western blot analysis of extracts from various cell lines using Stat3α (D1A5) XP ® Rabbit mAb (top), Stat3 (124H6) Mouse mAb #9139 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or PC-3 (right), using Stat3α (D1A5) XP ® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Stat3α (D1A5) XP ® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Confocal immunofluorescent analysis of HeLa cells, serum-starved (left) or treated with Human Interferon-α1 (hIFN-α1) #8927 (100 ng/mL, 30 min; right) using Stat3α (D1A5) XP ® Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Hep G2 cells starved overnight and treated with Human Interleukin-6 (hIL-6) #8904 (100 ng/ml, 30 min), and either 5 μl of Stat3α (D1A5) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP ® Human c-Fos Promoter Primers #4663, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.