Recombinant Rat IL‑13 (Catalog # 1945-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑13 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1945). The ND50 is typically 0.2-0.8 µg/mL.
Western blot shows 25 ng of Recombinant Rat IL‑13 (Catalog # 1945-RL), Recombinant Human IL‑13 (Catalog # 213-ILB) and Recombinant Mouse IL‑13 (Catalog # 413-ML). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Rat IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1945) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑13 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.