Western blot shows lysates of mouse embryo tissue and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Pentraxin 3/TSG‑14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2166) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pentraxin 3/TSG‑14 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.