Western Blot: Tubulin alpha-3E Antibody (2A8) [NBP2-02878] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tubulin alpha-3E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tubulin alpha-3E.
Immunocytochemistry/Immunofluorescence: Tubulin alpha-3E Antibody (2A8) [NBP2-02878] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tubulin alpha-3E.
Flow Cytometry: Tubulin alpha-3E Antibody (2A8) [NBP2-02878] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Tubulin alpha-3E antibody, and then analyzed by flow cytometry.