![Western Blot: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29668_HLCS-Antibody-Western-Blot-NBP2-32174-img0003.jpg)
Western Blot: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
![Immunoprecipitation: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for IP at 6 ug per reaction. HLCS was also immunoprecipitated by rabbit anti-HLCS antibody BL15481. For blotting immunoprecipitated HLCS, NBP2-32174 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/29669_HLCS-Antibody-Immunoprecipitation-NBP2-32174-img0002.jpg)
Immunoprecipitation: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for IP at 6 ug per reaction. HLCS was also immunoprecipitated by rabbit anti-HLCS antibody BL15481. For blotting immunoprecipitated HLCS, NBP2-32174 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.