Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor ® 488 Conjugate)

• Supplier: Cell Signaling Technology
• Catalog: 4854
• Prices: $299.00
• Sizes: 100 µl (50 tests)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: 2F9
• Applications: ICC/IF FC
• Species Reactivities: Human, Mouse, Rat, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6
• Description: Rabbit Monoclonal
• Gene: RPS6
• Conjugate: Alexa Fluor ® 488

Product images

Confocal immunofluorescent analysis of rat brain showing the hippocampus (left) and striatum (right) labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green), EGR1 Antibody (red) #4152, and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (blue) following ischemia with 30 minute reperfusion.

Confocal immunofluorescent analysis of two Gefinitib (Iressa) treated non-small cell lung cancer cell lines. HCC827 cells have the E746_A750 deletion in exon 19 of the EGFR gene, and are highly sensitive to Gefitinib. H1975 cells have the (T790M) mutation that confers Gefitinib-resistance. Both cell lines were treated and then double-labeled with Phospho-S6 Ribosomal Protein Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 and Phospho-Tyrosine Mouse mAb (Alexa Fluor® 647 Conjugate) #9415. Untreated HCC827 (A) and H1975 (C) cells show bright phospho-S6 (green) and phospho-tyrosine (red pseudocolor) label. Phospho-S6 and phospho-tyrosine signals dramatically decrease following Gefitinib treatment in HCC827 cells (B), with little or no change in the Gefitinib-resistant H1975 cells (D).

Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002, Wortmannin and U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (#4854).