Western blot analysis of immunoprecipitates from C6 cell lysates. Akt1 was immunoprecipitated with Akt1 (2H10) mAb #2967 (lane 1), Akt 2 was immunoprecipitated with Akt2 Antibody (lane 2), and specificity was confirmed by immunprecipitation with rabbit IgG (lane 3). Immunoblot was performed using Akt2 Antibody.
Western blot analysis of Akt1, Akt2, and Akt3 recombinant proteins, and extracts from C6 cells, using Akt2 Antibody.
Western blot analysis of extracts from Akt1 (lane 1), Akt2 (lane 2) and Akt3 (lane 3) knock-out mouse embryonic fibroblast cells and matched Akt2 wild-type mouse embryonic fibroblast cells (lane 4), using Akt2 Antibody (upper) and Akt Antibody #9272 (lower).