Formalin-fixed and paraffin embedded human laryngeal carcinoma labeled with Rabbit Anti-COX6c/Cytochrome C Oxidase subunit VIc Polyclonal Antibody, Unconjugated (bs-12925R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX6c/Cytochrome C Oxidase subunit VIc Polyclonal Antibody, Unconjugated (bs-12925R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.