Anti-PPP3CC (496-510) antibody produced in rabbit

Name	Anti-PPP3CC (496-510) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C9495
Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 496-510 of human PPP3CC
Description	Rabbit Polyclonal
Gene	PPP3CC
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-PPP3CC (496-510) (RB): <B>Cat. No. C9495</B>: Immunofluorescence of HUVEC cells using PPP3CC (496-510) (RB), <B>Cat. No. C9495</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.<BR/>Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-PPP3CC (496-510) (RB): Cat. No. C9495: Immunofluorescence of HUVEC cells using PPP3CC (496-510) (RB), Cat. No. C9495 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human PPP3CC was separated on SDS-PAGE, blotted with Anti-PPP3CC (496-510) (<B>Cat. No. C9495</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + PPP3CC immunizing peptide (human, 496-510)

Immunoblotting
Lysate of HEK-293T cells overexpressing human PPP3CC was separated on SDS-PAGE, blotted with Anti-PPP3CC (496-510) (Cat. No. C9495) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + PPP3CC immunizing peptide (human, 496-510)

<B>Chart</B><BR/>Anti-PPP3CC (496-510) (RB): <B>Cat. No. C9495</B>: Intensity analysis of Anti-PPP3CC (496-510) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-PPP3CC (496-510) (RB): Cat. No. C9495: Intensity analysis of Anti-PPP3CC (496-510) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Calcineurin expression and activity is regulated by the intracellular redox - Calcineurin expression and activity is regulated by the intracellular redox

Alba G, Santa-Maria C, Reyes-Quiroz ME, El Bekay R, Geniz I, Martin-Nieto J, Pintado E, Sobrino F. J Endocrinol. 2012 Sep;214(3):399-408. Epub 2012 Jun 27.

[Expression profile of calcineurin pathway genes in myocardium tissues in - [Expression profile of calcineurin pathway genes in myocardium tissues in

Polovlkova OG, Makeeva OA, Lezhnev AA, Goncharova IA, Kulish EV, Shipulin VM, Puzyrev VP. Mol Biol (Mosk). 2013 May-Jun;47(3):433-40.