ImmunofluorescenceAnti-RFC5 (6-20) :
Cat. No. SAB1100567: Immunofluorescence of HUVEC cells using RFC5 (6-20) , Cat. No. SAB1100567 (red) at a 1:400 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ImmunoblottingLysate of HEK-293T cells overexpressing human RFC5 was separated on SDS-PAGE, blotted with Anti-RFC5 (6-20) (
Cat. No. SAB1100567) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:500 + RFC5 immunizing peptide (human, 6-20)
3. Antibody dilution 1:1,000