Anti-AP2A2 (631-643) antibody produced in rabbit

Name	Anti-AP2A2 (631-643) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	A9858
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 631-643 of human AP2A2
Description	Rabbit Polyclonal
Gene	AP2A2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-AP2A2 (631-643): <B>Cat. No. A9858</B>: Immunofluorescence of HUVEC cells using AP2A2 (631-643) (RB),<B>Cat. No. A9858</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-AP2A2 (631-643): Cat. No. A9858: Immunofluorescence of HUVEC cells using AP2A2 (631-643) (RB),Cat. No. A9858 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human AP2A2 was separated on SDS-PAGE, blotted with Anti-AP2A2 (631-643) (<B>Cat. No. A9858</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500

Immunoblotting
Lysate of HEK-293T cells overexpressing human AP2A2 was separated on SDS-PAGE, blotted with Anti-AP2A2 (631-643) (Cat. No. A9858) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500

<B>Chart</B><BR/>Anti-AP2A2 (631-643): <B>Cat. No. A9858</B>: Intensity analysis of Anti-AP2A2 (631-643) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-AP2A2 (631-643): Cat. No. A9858: Intensity analysis of Anti-AP2A2 (631-643) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Mutations in AP2S1 cause familial hypocalciuric hypercalcemia type 3. - Mutations in AP2S1 cause familial hypocalciuric hypercalcemia type 3.

Nesbit MA, Hannan FM, Howles SA, Reed AA, Cranston T, Thakker CE, Gregory L, Rimmer AJ, Rust N, Graham U, Morrison PJ, Hunter SJ, Whyte MP, McVean G, Buck D, Thakker RV. Nat Genet. 2013 Jan;45(1):93-7.