Anti-DBT (246-260) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: D6570
• Prices: $328.50
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 246-260 of human DBT
• Description: Rabbit Polyclonal
• Gene: DBT
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-DBT (246-260), Cat. No. D6570: Immunofluorescence of HUVEC cells using Anti-DBT (246-260), D6570 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunoblotting
Lysate of HEK-293T cells overexpressing human FLAG-DBT fusion protein was separated on SDS-PAGE, blotted with Anti-DBT (246-260) (Cat. No. D6570) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000
2. Negative control (only secondary antibody)
3. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μg/mL

Immunofluorescence Graph
Anti-DBT (246-260), Cat. No. D6570: Intensity analysis of Anti-DBT (246-260) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

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