Anti-ATP6AP1 (451-465) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: A1486
• Prices: $328.50
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 451-465 of human ATP6AP1
• Description: Rabbit Polyclonal
• Gene: ATP6AP1
• Conjugate: unconjugated

Product images

Immunoblotting
Lysate of HEK-293T cells overexpressing human ATP6AP1 was separated on SDS-PAGE, blotted with Anti-ATP6AP1 (451-465) (Cat. No. A1486) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250

Immunofluorescence
Anti-ATP6AP1 (451-465): Cat. No. A1486: Immunofluorescence of HUVEC cells using ATP6AP1 (451-465), Cat. No. A1486 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Chart
Anti-ATP6AP1 (451-465): Cat. No. A1486: Intensity analysis of Anti-ATP6AP1 (451-465) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

V-ATPase subunit ATP6AP1 (Ac45) regulates osteoclast differentiation, - V-ATPase subunit ATP6AP1 (Ac45) regulates osteoclast differentiation,

Yang DQ, Feng S, Chen W, Zhao H, Paulson C, Li YP. J Bone Miner Res. 2012 Aug;27(8):1695-707.

The vacuolar-ATPase complex regulates retinoblast proliferation and survival, - The vacuolar-ATPase complex regulates retinoblast proliferation and survival,

Nuckels RJ, Ng A, Darland T, Gross JM. Invest Ophthalmol Vis Sci. 2009 Feb;50(2):893-905.