ImmunofluorescencePC3 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Mouse Anti-Tensin 3 Clone: TN-17 (
Cat. No. SAB4200416). The antibody was developed using Goat Anti-Mouse IgG, Cy3
™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingSW620 cell extract was separated on SDS-PAGE and probed with Mouse Anti-Tensin 3 Clone: TN-17 (
Cat. No. SAB4200416). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. 1 μg/mL antibody
2. 1 μg/mL antibody + Tensin 3 immunizing peptide (human, 401-418)
3. Negative control
ImmunoblottingCell extracts were separated on SDS-PAGE and probed with 1 μg/mL Mouse Anti-Tensin 3 Clone: TN-17 (
Cat. No. SAB4200416). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. MDBK; 2. LA4; 3. CFB; 4. F9; 5. COS7; 6. MDCK; 7. GH3; 8. BHK; 9. A549