All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1/1000 dilutionLane 1 : Rat brain stem lysateLane 2 : Rat brain stem lysate with the phosphopeptide used as the antigenObserved band size : 29 kDa (why is the actual band size different from the predicted?)
ab30554 staining 14-3-3 (phospho S58) in Mouse hippocampal tissue by Immunohistochemistry (Frozen sections). ab30554 is green, red staining is GFAP and nuclei are stained blue with DAPI.
![ab30554 staining 14-3-3 (phospho S58) in human U2OS cells by Immunocytochemistry/ Immunofluorescence.U2OS cells were plated on coverslips and fixed by sequential incubations with 4% formaldehyde in PBS without calcium and magnesium [PBS(-)], 0.1% Triton X-100 in PBS(-) and 0.05% Tween-20 in PBS(-), each for 10 minutes at room temperature. After being washed, cells were incubated with ab30554, followed by incubation with Alexa Fluor 488–conjugated anti-rabbit IgG in TBST (100 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.05% Tween-20) containing 5% FBS.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/78_14-3-3-Primary-antibodies-ab30554-3.jpg)
ab30554 staining 14-3-3 (phospho S58) in human U2OS cells by Immunocytochemistry/ Immunofluorescence.U2OS cells were plated on coverslips and fixed by sequential incubations with 4% formaldehyde in PBS without calcium and magnesium [PBS(-)], 0.1% Triton X-100 in PBS(-) and 0.05% Tween-20 in PBS(-), each for 10 minutes at room temperature. After being washed, cells were incubated with ab30554, followed by incubation with Alexa Fluor 488–conjugated anti-rabbit IgG in TBST (100 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.05% Tween-20) containing 5% FBS.
All lanes : Anti-14-3-3 (phospho S58) antibody (ab30554) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate (20ug)Lane 3 : Mouse brain homogenate (20ug)SecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 28 kDa (why is the actual band size different from the predicted?)Exposure time : 1 minute