Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling 14-3-3 sigma with ab86380 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-14-3-3 sigma antibody (ab86380) at 0.1 µg/mlLane 1 : Hela whole cell lysate at 50 µgLane 2 : Hela whole cell lysate at 15 µgLane 3 : Hela whole cell lysate at 5 µgdeveloped using the ECL technique
Detection of 14-3-3 sigma by Western blot of Immunprecipitate. ab86380, at 1 µg/ml, staining 14-3-3 sigma in HeLa whole cell lysate immunoprecipitated using ab86380 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.