Anti-14-3-3 Tau antibody [3B9]
| Name | Anti-14-3-3 Tau antibody [3B9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab10439 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 3B9 |
| Applications | FC IP WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Recombinant full length protein |
| Description | Mouse Monoclonal |
| Gene | YWHAQ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
14-3-3 Tau was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to 14-3-3 Tau and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10439.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 31kDa, non specific band - 26kDa: We are unsure as to the identity of this extra band; 14-3-3 Tau
ICC/IF image of ab10439 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10439, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![All lanes : Anti-14-3-3 Tau antibody [3B9] (ab10439)Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/240_14-3-3-Tau-Primary-antibodies-ab10439-3.jpg)
All lanes : Anti-14-3-3 Tau antibody [3B9] (ab10439)Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![Overlay histogram showing SH-SY5Y cells stained with ab10439 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10439, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/241_14-3-3-Tau-Primary-antibodies-ab10439-4.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab10439 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10439, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
14-3-3theta protects against neurotoxicity in a cellular Parkinson's disease - 14-3-3theta protects against neurotoxicity in a cellular Parkinson's disease
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Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics - Brainstem deficiency of the 14-3-3 regulator of serotonin synthesis: a proteomics
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Quantitative protein expression profiling of 14-3-3 isoforms in human renal - Quantitative protein expression profiling of 14-3-3 isoforms in human renal
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14-3-3zeta/tau heterodimers regulate Slingshot activity in migrating - 14-3-3zeta/tau heterodimers regulate Slingshot activity in migrating
Kligys K, Yao J, Yu D, Jones JC. Biochem Biophys Res Commun. 2009 Jun 12;383(4):450-4. doi:
Proteomic analysis of hypoxia-induced responses in the syncytialization of human - Proteomic analysis of hypoxia-induced responses in the syncytialization of human
Hu R, Jin H, Zhou S, Yang P, Li X. Placenta. 2007 May-Jun;28(5-6):399-407. Epub 2006 Nov 13.
Automated quantification tool for high-throughput proteomics using stable isotope - Automated quantification tool for high-throughput proteomics using stable isotope
Wang G, Wu WW, Pisitkun T, Hoffert JD, Knepper MA, Shen RF. Anal Chem. 2006 Aug 15;78(16):5752-61.