Anti-3-Nitrotyrosine antibody [7A12AF6]

Name	Anti-3-Nitrotyrosine antibody [7A12AF6]
Supplier	Abcam
Catalog	ab110282
Prices	$390.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone	7A12AF6
Applications	ICC/IF ICC/IF FC WB IP ELISA
Species Reactivities	Mouse, Rat, Bovine, Human
Antigen	Nitrated KLH (Keyhole Limpet Hemocyanin)
Description	Mouse Monoclonal
Conjugate	Unconjugated
Supplier Page	Shop

Product images

HL-60 cells were stained with 1 µg/ml ab110282 following treatment with 2 mM peroxynitrite (blue) or vehicle control (red). No primary antibody control is shown in black. Peroxynitrite modifies tyrosine residues to 3-nitrotyrosine.

Immunocytochemistry image of ab110282 stained Human HeLa cells (A) and fibroblast cells (B, C). Cells grown on slides were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). Slides were treated with/without 1 mM peroxynitrite to modify exposed tyrosines to 3-nitrotyrosine. Slides were blocked and incubated with tab110282 at 2 µg/ml overnight at 4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. For reference, the mitochondria (red) were identified by HSP60 / Alexa Fluor® 594 and DAPI was used to stain the cell nuclei (blue). HeLa cells (A) and fibroblast cells (B) show surface modification of tyrosine to 3-nitrotyrosine after exposure to peroxynitrite. While (C) unexposed fibroblast cells show no modification.

All lanes : Anti-3-Nitrotyrosine antibody [7A12AF6] (ab110282) at 1 µg/mlLane 1 : Bovine heart mitochondriaLane 2 : Bovine heart mitochondria - nitratedLane 3 : BSALane 4 : BSA - nitrated

Product References

Establishing 3-nitrotyrosine as a biomarker for the vasculopathy of Fabry - Establishing 3-nitrotyrosine as a biomarker for the vasculopathy of Fabry

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The impact of ageing on adipose structure, function and vasculature in the B6D2F1 - The impact of ageing on adipose structure, function and vasculature in the B6D2F1

Donato AJ, Henson GD, Hart CR, Layec G, Trinity JD, Bramwell RC, Enz RA, Morgan RG, Reihl KD, Hazra S, Walker AE, Richardson RS, Lesniewski LA. J Physiol. 2014 Sep 15;592(Pt 18):4083-96.

Obesity-induced endothelial dysfunction is prevented by deficiency of P-selectin - Obesity-induced endothelial dysfunction is prevented by deficiency of P-selectin

Wang H, Luo W, Wang J, Guo C, Wang X, Wolffe SL, Bodary PF, Eitzman DT. Diabetes. 2012 Dec;61(12):3219-27.