Anti-53BP1 antibody
| Name | Anti-53BP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21083 |
| Prices | $394.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | In situ hybridisation WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide corresponding to the carboxy-terminus of h53BP1, conjugated to a carrier protein |
| Description | Rabbit Polyclonal |
| Gene | TP53BP1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab21083 (2µg/ml) staining 53BP1 in human Brain: Cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is ubiquitous nuclear staining throughout.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
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