Anti-5HT7 Receptor antibody
| Name | Anti-5HT7 Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab61562 |
| Prices | $376.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Horse, Guinea Pig, Hamster, Bovine, Dog, Pig, Monkey, Ape, Marmoset, Bat |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to 19 amino acids from the 3rd cytoplasmic domain of Human 5HT7 Receptor |
| Description | Rabbit Polyclonal |
| Gene | Htr7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue, staining 5HT7 Receptor with ab61562 at 10 µg/ml.
ICC/IF image of ab61562 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61562, 1:200) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.