Anti-67kDa Laminin Receptor antibody [MLuC5]
| Name | Anti-67kDa Laminin Receptor antibody [MLuC5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3099 |
| Prices | $398.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | MLuC5 |
| Applications | B/N IHC-F WB FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Small cell lung carcinoma N592 live cells |
| Description | Mouse Monoclonal |
| Gene | RPSA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab3099 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3099, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Anti-67kDa Laminin Receptor antibody [MLuC5] (ab3099) at 1/1000 dilution + Mouse pancreatic cancer tissue lysate at 35 µgSecondaryHRP-conjugated Sheep anti-Mouse polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/710_67kDa-Laminin-Receptor-Primary-antibodies-ab3099-12.jpg)
Anti-67kDa Laminin Receptor antibody [MLuC5] (ab3099) at 1/1000 dilution + Mouse pancreatic cancer tissue lysate at 35 µgSecondaryHRP-conjugated Sheep anti-Mouse polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab3099 staining 67kDa Laminin Receptor in human SH-SY5Y cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/400) for 1 hour at 20°C. An Allophycocyanin-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.Gating Strategy: isotype population (shown in white).See Abreview
![Overlay histogram showing MCF7 cells stained with ab3099 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3099, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/712_67kDa-Laminin-Receptor-Primary-antibodies-ab3099-15.jpg)
Overlay histogram showing MCF7 cells stained with ab3099 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3099, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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Polyphenon [corrected] E enhances the antitumor immune response in neuroblastoma - Polyphenon [corrected] E enhances the antitumor immune response in neuroblastoma
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Orihuela CJ, Mahdavi J, Thornton J, Mann B, Wooldridge KG, Abouseada N, Oldfield NJ, Self T, Ala'Aldeen DA, Tuomanen EI. J Clin Invest. 2009 Jun;119(6):1638-46.
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Effects of down-regulating the Id genes in human colorectal cancer cells on early - Effects of down-regulating the Id genes in human colorectal cancer cells on early
Okaji Y, Tsuno NH, Kitayama J, Sakurai D, Tsuchiya N, Saito S, Takegami K, Tsuchiya T, Kawai K, Yazawa K, Asakage M, Yoneyama S, Yamada J, Tokunaga K, Takahashi K, Nagawa H. Eur J Cancer. 2006 Mar;42(5):668-73. Epub 2006 Jan 19.