Anti-68kDa Neurofilament antibody [DA2]
| Name | Anti-68kDa Neurofilament antibody [DA2] |
|---|---|
| Supplier | Abcam |
| Catalog | ab7255 |
| Prices | $390.00 |
| Sizes | 250 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | DA2 |
| Applications | FC ICC/IF ICC/IF WB IHC-F IHC-P IHC-F |
| Species Reactivities | Rat, Human, Bird, Mammalian |
| Antigen | A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H |
| Description | Mouse Monoclonal |
| Gene | NEFL |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Lane 1 : Coomassie Blue stainLane 2 : Heavy neurofilament antibodyLane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)Lane 4 : Anti-68kDa Neurofilament antibody [DA2] (ab7255)Lane 5 : alpha Internexin antibodyLane 6 : GFAP antibodyLane 1 : Cytoskeletal homogenates of rat spinal cordLane 2 : Cytoskeletal homogenates of rat spinal cordLane 3 : Cytoskeletal homogenates of rat spinal cordLane 4 : Cytoskeletal homogenates of rat spinal cordLane 5 : Cytoskeletal homogenates of rat spinal cordLane 6 : Cytoskeletal homogenates of rat spinal cord](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/739_ab7255_1.jpg)
Lane 1 : Coomassie Blue stainLane 2 : Heavy neurofilament antibodyLane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)Lane 4 : Anti-68kDa Neurofilament antibody [DA2] (ab7255)Lane 5 : alpha Internexin antibodyLane 6 : GFAP antibodyLane 1 : Cytoskeletal homogenates of rat spinal cordLane 2 : Cytoskeletal homogenates of rat spinal cordLane 3 : Cytoskeletal homogenates of rat spinal cordLane 4 : Cytoskeletal homogenates of rat spinal cordLane 5 : Cytoskeletal homogenates of rat spinal cordLane 6 : Cytoskeletal homogenates of rat spinal cord
IHC image of ab7255 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7255, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Overlay histogram showing SH-SY5Y cells stained with ab7255 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7255, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/741_68kDa-Neurofilament-Primary-antibodies-ab7255-2.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab7255 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7255, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
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Subjects harboring presenilin familial Alzheimer's disease mutations exhibit - Subjects harboring presenilin familial Alzheimer's disease mutations exhibit
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Gamma-diketone axonopathy: analyses of cytoskeletal motors and highways in CNS - Gamma-diketone axonopathy: analyses of cytoskeletal motors and highways in CNS
Zhang L, Gavin T, DeCaprio AP, LoPachin RM. Toxicol Sci. 2010 Sep;117(1):180-9.
Identification of a tektin-like protein associated with neurofilaments in the - Identification of a tektin-like protein associated with neurofilaments in the
Edson KJ, Linck RW, Letourneau PC. J Neurosci Res. 1991 Sep;30(1):105-15.