Anti-6X His tag® antibody [4D11]

• Supplier: Abcam
• Catalog: ab5000
• Prices: $400.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 4D11
• Applications: FC DB ELISA WB IP ICC/IF ICC/IF
• Antigen: Recombinant malaria protein TRAP fused at the amino-terminal end with six His tag
• Description: Mouse Monoclonal
• Conjugate: Unconjugated

Product images

All lanes : Anti-6X His tag® antibody [4D11] (ab5000) at 1/2000 dilutionLane 1 : Lysates prepared from HEK-293 cellsLane 2 : Lysates prepared from HEK-293 cells transfected with Beta3-His (CACNB3)SecondaryHRP-conjugated goat anit-mouse IgG polyclonal at 1/5000 dilutionThis image is courtesy of an Abreview submitted by Dr Vladimir MilenkovicSee Abreview

ab5000 Immunoprecipitate in human HEK293 whole cell lysate. 25µg of cell lysate incubated with primary antibody (undiluted) and matrix (Protein G) for 16 hours at 4°C. For western blotting an undiluted HRP-Goat anti-rabbit IgG polyclonal was used. Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab Lane 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)Lane 2. IP with anti His Ab Lane 3. Non bound fractionSee Abreview

Anti-6X His tag® antibody [4D11] (ab5000) at 1/1000 dilution + 6X His Tagged Protein at 1 µgSecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 30 seconds

ab5000 staining Human HEK 293 cells transfected with CACNB3-His by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody (1/250 in 1% goat serum, 0.1% TX100, 1X PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody (1/500) was used as the secondary.  DAPI was used for nuclei staining (blue).See Abreview

Product References

Human T cell leukemia virus type 2 tax-mediated NF-kappaB activation involves a - Human T cell leukemia virus type 2 tax-mediated NF-kappaB activation involves a

Journo C, Bonnet A, Favre-Bonvin A, Turpin J, Vinera J, Cote E, Chevalier SA, Kfoury Y, Bazarbachi A, Pique C, Mahieux R. J Virol. 2013 Jan;87(2):1123-36.

Human MTH3 (NUDT18) protein hydrolyzes oxidized forms of guanosine and - Human MTH3 (NUDT18) protein hydrolyzes oxidized forms of guanosine and

Takagi Y, Setoyama D, Ito R, Kamiya H, Yamagata Y, Sekiguchi M. J Biol Chem. 2012 Jun 15;287(25):21541-9.

A genome-wide synthetic dosage lethality screen reveals multiple pathways that - A genome-wide synthetic dosage lethality screen reveals multiple pathways that

Liu C, van Dyk D, Li Y, Andrews B, Rao H. BMC Biol. 2009 Nov 12;7:75.

Investigation of the role of SRC in capacitation-associated tyrosine - Investigation of the role of SRC in capacitation-associated tyrosine

Mitchell LA, Nixon B, Baker MA, Aitken RJ. Mol Hum Reprod. 2008 Apr;14(4):235-43.

Serum- and glucocorticoid-inducible kinase 1 enhances zif268 expression through - Serum- and glucocorticoid-inducible kinase 1 enhances zif268 expression through

Tyan SW, Tsai MC, Lin CL, Ma YL, Lee EH. J Neurochem. 2008 May;105(3):820-32. Epub 2007 Dec 13.

The cytotoxic necrotizing factors from Yersinia pseudotuberculosis and from - The cytotoxic necrotizing factors from Yersinia pseudotuberculosis and from

Blumenthal B, Hoffmann C, Aktories K, Backert S, Schmidt G. Infect Immun. 2007 Jul;75(7):3344-53. Epub 2007 Apr 16.

Alpha-synuclein in the nucleus accumbens induces changes in cocaine behaviour in - Alpha-synuclein in the nucleus accumbens induces changes in cocaine behaviour in

Boyer F, Dreyer JL. Eur J Neurosci. 2007 Nov;26(10):2764-76.

Regulation of the polarity kinases PAR-1/MARK by 14-3-3 interaction and - Regulation of the polarity kinases PAR-1/MARK by 14-3-3 interaction and

Goransson O, Deak M, Wullschleger S, Morrice NA, Prescott AR, Alessi DR. J Cell Sci. 2006 Oct 1;119(Pt 19):4059-70. Epub 2006 Sep 12.

14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and - 14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and

Al-Hakim AK, Goransson O, Deak M, Toth R, Campbell DG, Morrice NA, Prescott AR, Alessi DR. J Cell Sci. 2005 Dec 1;118(Pt 23):5661-73.

Epitope mapping of cytochrome P450 cholesterol side-chain cleavage enzyme by sera - Epitope mapping of cytochrome P450 cholesterol side-chain cleavage enzyme by sera

Liiv I, Teesalu K, Peterson P, Clemente MG, Perheentupa J, Uibo R. Eur J Endocrinol. 2002 Jan;146(1):113-9.