Anti-6X His tag® antibody [HIS.H8]
| Name | Anti-6X His tag® antibody [HIS.H8] |
|---|---|
| Supplier | Abcam |
| Catalog | ab18184 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | HIS.H8 |
| Applications | ICC/IF ICC/IF ELISA DB WB IP EMSA |
| Antigen | 6x His synthetic peptide |
| Description | Mouse Monoclonal |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence (red) with ab18184 on His-tag fusion protein in 293 cells.Left hand panel: untransfected controlRight hand panel: transfected.Counterstained with DAPI (blue).
![All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilutionLane 1 : 293 cells, untransfected controlLane 2 : 293 cells transfected with His-tagged vector](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/766_ab18184_3.jpg)
All lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilutionLane 1 : 293 cells, untransfected controlLane 2 : 293 cells transfected with His-tagged vector
![Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution + Conditioned medium from transfected HEK293 cells which produce recombinant secretory protein with a 6XHis-Tag at 5 µgSecondaryAn HRP-conjugated Horse anti mouse polyclonal at 1/5000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/767_6X-His-tag-Primary-antibodies-ab18184-2.jpg)
Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution + Conditioned medium from transfected HEK293 cells which produce recombinant secretory protein with a 6XHis-Tag at 5 µgSecondaryAn HRP-conjugated Horse anti mouse polyclonal at 1/5000 dilutionThis image is courtesy of an anonymous AbreviewSee Abreview
ab18184 at a 1/1000 dilution staining 6X His tag in transfected human HEK293 cells by Immunocytochemistry/ Immunofluorescence incubated for 12 hours at 22°C. PFA fixed. Permeabilized using 0.1% Triton X-100 in PBS. Blocked using 3% BSA for 1 hour at 22°C. Secondary used at a 1/1000 dilution polyclonal Goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 (green). DAPI staining (blue).See Abreview
Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 at 1/100 dilution.Lane 1 : MW Ladder (from top 120, 85, 50, 34, 25, 19 kDa) Lanes 2-5 : Immunoprecipitation of His/FLAG tagged PDCD5 using ab18184 from mixture of ~50 ng purified His/FLAG tagged PDCD5 and 15 µL RRL in 300 µL buffer. Lanes 6-7 : Purified PDCD5 from E. coli with ~60-70 ng protein. The lowest band in each lane is ~ 20 kDa and is PDCD5. The upper bands in lanes 2-5 are the heavy chain (~50 kDa, runs as doublet) and light chain (~25 or 26 kDa). Western Blot was performed with a mouse anti-FLAG primary antibody at 1/2000 dilution. An IRDye®800CW-conjugated goat anti-mouse IgG was used as a secondary antibody.See Abreview
Product References
The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor - The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor
Booy EP, Howard R, Marushchak O, Ariyo EO, Meier M, Novakowski SK, Deo SR, Dzananovic E, Stetefeld J, McKenna SA. Nucleic Acids Res. 2014 Mar;42(5):3346-61.
Protein interaction screening for the ankyrin repeats and suppressor of cytokine - Protein interaction screening for the ankyrin repeats and suppressor of cytokine
Andresen CA, Smedegaard S, Sylvestersen KB, Svensson C, Iglesias-Gato D, Cazzamali G, Nielsen TK, Nielsen ML, Flores-Morales A. J Biol Chem. 2014 Jan 24;289(4):2043-54.
Sentrin/SUMO specific proteases as novel tissue-selective modulators of vitamin D - Sentrin/SUMO specific proteases as novel tissue-selective modulators of vitamin D
Lee WP, Jena S, Doherty D, Ventakesh J, Schimdt J, Furmick J, Widener T, Lemau J, Jurutka PW, Thompson PD. PLoS One. 2014 Feb 20;9(2):e89506.
Analysis of factors that affect FlgM-dependent type III secretion for protein - Analysis of factors that affect FlgM-dependent type III secretion for protein
Guo S, Alshamy I, Hughes KT, Chevance FF. J Bacteriol. 2014 Jul;196(13):2333-47.
N-terminal phosphorylation of phosphoprotein of vesicular stomatitis virus is - N-terminal phosphorylation of phosphoprotein of vesicular stomatitis virus is
Chen L, Zhang S, Banerjee AK, Chen M. J Virol. 2013 Mar;87(6):3177-86.
ORF11 protein interacts with the ORF9 essential tegument protein in - ORF11 protein interacts with the ORF9 essential tegument protein in
Che X, Oliver SL, Reichelt M, Sommer MH, Haas J, Rovis TL, Arvin AM. J Virol. 2013 May;87(9):5106-17.
Molecular cloning, overexpression, and an efficient one-step purification of - Molecular cloning, overexpression, and an efficient one-step purification of
Tartaglia LJ, Bennett A, Plattner AS, Muzyczka N, Ling C, Srivastava A, Agbandje-McKenna M. Protein Expr Purif. 2013 Nov;92(1):21-8.
Smad6 inhibits non-canonical TGF-beta1 signalling by recruiting the - Smad6 inhibits non-canonical TGF-beta1 signalling by recruiting the
Jung SM, Lee JH, Park J, Oh YS, Lee SK, Park JS, Lee YS, Kim JH, Lee JY, Bae YS, Koo SH, Kim SJ, Park SH. Nat Commun. 2013;4:2562.
Binding of G-quadruplexes to the N-terminal recognition domain of the RNA - Binding of G-quadruplexes to the N-terminal recognition domain of the RNA
Meier M, Patel TR, Booy EP, Marushchak O, Okun N, Deo S, Howard R, McEleney K, Harding SE, Stetefeld J, McKenna SA. J Biol Chem. 2013 Dec 6;288(49):35014-27.
Dynamics of major histocompatibility complex class I association with the human - Dynamics of major histocompatibility complex class I association with the human
Panter MS, Jain A, Leonhardt RM, Ha T, Cresswell P. J Biol Chem. 2012 Sep 7;287(37):31172-84.