Anti-6X His tag® antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab9108
• Prices: $403.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IP WB IHC-P ICC/IF ICC/IF ELISA ChIP FC
• Antigen: Synthetic peptide: HHHHHH conjugated to KLH
• Description: Rabbit Polyclonal
• Conjugate: Unconjugated

Product images

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).

All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/4000 dilutionLane 1 : HeLa whole cell lysate - transfected with empty vectorLane 2 : HeLa whole cell lysate - transfected with His-tagged geneLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 35 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsThis image is courtesy of an anonymous AbreviewSee Abreview

Use of ab9108 in western blotting at 1:1000 against Epitope Tag Positive Control lysate (ab4524). Lane 1: 2 µl lysate Lane 2: 1 µl lysate Lane 3: 0.5 µl lysate Lane 4: 0.25 µl lysate Use of ab9108 in western blotting at 1:1000 against E.coli Positive Control (Escherichia coli ) Whole Cell Lysate - expressing 11 different epitope tag (ab5395). Lane 1: 2 µl lysate Lane 2: 1 µl lysate Lane 3: 0.5 µl lysate Lane 4: 0.25 µl lysate

All lanes : Anti-6X His tag® antibody - ChIP Grade (ab9108) at 1/1000 dilutionLane 1 : 10ug non-transformed lysateLane 2 : 10ug non-transformed lysate + 1mM IPTGLane 3 : 10ug E.coli Lysate harbouring His-tag-RelA NTD, non-inducedLane 4 : 10ug Induction of His-tag RelA NTD 1 mM IPTGLane 5 : 10ug Induction of His-tag RelA NTD 1 mM IPTGSecondaryHRP conjugated goat anti-rabbit IgGdeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 37 kDa (why is the actual band size different from the predicted?)Additional bands at : 12 kDa (possible non-specific binding).Exposure time : 1 minuteThis image is courtesy of an Abreview.See Abreview

Product References

Identification of the alternative sigma factor SigX regulon and its implications - Identification of the alternative sigma factor SigX regulon and its implications

Blanka A, Schulz S, Eckweiler D, Franke R, Bielecka A, Nicolai T, Casilag F, Duvel J, Abraham WR, Kaever V, Haussler S. J Bacteriol. 2014 Jan;196(2):345-56.

Tissue inhibitor of matrix metalloproteinases-1 loaded poly(lactic-co-glycolic - Tissue inhibitor of matrix metalloproteinases-1 loaded poly(lactic-co-glycolic

Chaturvedi M, Molino Y, Sreedhar B, Khrestchatisky M, Kaczmarek L. Int J Nanomedicine. 2014 Jan 20;9:575-88.

A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes. - A role for Na+,K+-ATPase alpha1 in regulating Rab27a localisation on melanosomes.

Booth AE, Tarafder AK, Hume AN, Recchi C, Seabra MC. PLoS One. 2014 Jul 22;9(7):e102851.

Self-regulation of exopolysaccharide production in Bacillus subtilis by a - Self-regulation of exopolysaccharide production in Bacillus subtilis by a

Elsholz AK, Wacker SA, Losick R. Genes Dev. 2014 Aug 1;28(15):1710-20.

Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the - Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the

Crispin M, Harvey DJ, Bitto D, Halldorsson S, Bonomelli C, Edgeworth M, Scrivens JH, Huiskonen JT, Bowden TA. J Virol. 2014 Sep 1;88(17):10244-51.

Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human - Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human

Brouxhon SM, Kyrkanides S, Teng X, O'Banion MK, Clarke R, Byers S, Ma L. Mol Carcinog. 2014 Nov;53(11):893-906.

LMX1B is part of a transcriptional complex with PSPC1 and PSF. - LMX1B is part of a transcriptional complex with PSPC1 and PSF.

Hoekstra EJ, Mesman S, de Munnik WA, Smidt MP. PLoS One. 2013;8(1):e53122.

Specification of dopaminergic subsets involves interplay of En1 and Pitx3. - Specification of dopaminergic subsets involves interplay of En1 and Pitx3.

Veenvliet JV, Dos Santos MT, Kouwenhoven WM, von Oerthel L, Lim JL, van der Linden AJ, Koerkamp MJ, Holstege FC, Smidt MP. Development. 2013 Aug;140(16):3373-84.

Reward learning requires activity of matrix metalloproteinase-9 in the central - Reward learning requires activity of matrix metalloproteinase-9 in the central

Knapska E, Lioudyno V, Kiryk A, Mikosz M, Gorkiewicz T, Michaluk P, Gawlak M, Chaturvedi M, Mochol G, Balcerzyk M, Wojcik DK, Wilczynski GM, Kaczmarek L. J Neurosci. 2013 Sep 4;33(36):14591-600.

Elucidation of the role of LMO2 in human erythroid cells. - Elucidation of the role of LMO2 in human erythroid cells.

Inoue A, Fujiwara T, Okitsu Y, Katsuoka Y, Fukuhara N, Onishi Y, Ishizawa K, Harigae H. Exp Hematol. 2013 Dec;41(12):1062-76.e1.