Anti-ABAT antibody [EPR4433]
| Name | Anti-ABAT antibody [EPR4433] |
|---|---|
| Supplier | Abcam |
| Catalog | ab108249 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR4433 |
| Applications | WB IP IHC-P FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues in Human ABAT |
| Blocking Peptide | ABAT peptide |
| Description | Rabbit Monoclonal |
| Gene | ABAT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-ABAT antibody [EPR4433] (ab108249)Lane 1 : HepG2 cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : Human fetal liver tissue lysateLane 4 : rat brain tissue lysateLane 5 : rat kidney tissue lysateLane 6 : mouse brain tissue lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/956_ABAT-Primary-antibodies-ab108249-1.JPG)
All lanes : Anti-ABAT antibody [EPR4433] (ab108249)Lane 1 : HepG2 cell lysateLane 2 : SH-SY5Y cell lysateLane 3 : Human fetal liver tissue lysateLane 4 : rat brain tissue lysateLane 5 : rat kidney tissue lysateLane 6 : mouse brain tissue lysate
ab108249, at 1/250 dilution, staining ABAT in Human liver tissue by immunohistochemistry.
Overlay histogram showing HepG2 cells stained with ab108249 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108249, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Synchronization by food access modifies the daily variations in expression and - Synchronization by food access modifies the daily variations in expression and
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GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's - GABA from reactive astrocytes impairs memory in mouse models of Alzheimer's
Jo S, Yarishkin O, Hwang YJ, Chun YE, Park M, Woo DH, Bae JY, Kim T, Lee J, Chun H, Park HJ, Lee da Y, Hong J, Kim HY, Oh SJ, Park SJ, Lee H, Yoon BE, Kim Y, Jeong Y, Shim I, Bae YC, Cho J, Kowall NW, Ryu H, Hwang E, Kim D, Lee CJ. Nat Med. 2014 Aug;20(8):886-96.