Anti-ABCD1 antibody [EPR15929]

• Supplier: Abcam
• Catalog: ab197013
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15929
• Applications: WB ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human ABCD1 aa 650 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: ABCD1
• Conjugate: Unconjugated

Product images

All lanes : Anti-ABCD1 antibody [EPR15929] (ab197013) at 1/10000 dilutionLane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 3 : Human fetal liver lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-ABCD1 antibody [EPR15929] (ab197013) at 1/10000 dilution + JAR (Human placenta choriocarcinoma cell line) whole cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-ABCD1 antibody [EPR15929] (ab197013) at 1/10000 dilutionLane 1 : C6 (Rat glial tumor cells) whole cell lysateLane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ABCD1 with ab197013 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab197013 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.