All lanes : Anti-ABCF1 antibody [EPR16067] (ab191076) at 1/50000 dilutionLane 1 : HeLa lysateLane 2 : 293T lysateLane 3 : K562 lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of paraformaldehyde-fixed Raw264.7 cells labeling ABCF1 with ab191076 at 1/100, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue). Negative controls also shown.
Flow Cytometrical analysis of 293T cells labeling ABCF1 with ab191076 at 1/60 compared to a Isotype control. FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ABCF1 with ab191076 at 1/250. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Secondary-only negative control also shown.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ABCF1 with ab191076 at 1/250. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Secondary-only negative control also shown.