Anti-ACAA1 antibody [2H6BE7]

• Supplier: Abcam
• Catalog: ab110289
• Prices: $359.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 2H6BE7
• Applications: ICC/IF FC IP WB ELISA
• Species Reactivities: Mouse, Rat, Human
• Antigen: Human liver mitochondria
• Description: Mouse Monoclonal
• Gene: ACAA1
• Conjugate: Unconjugated

Product images

ACAA1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 44kDa; ACAA1

ab110289, at 5 µg/mL, staining ACAA1 in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).

ab110289, at 1 µg/mL, staining ACAA1 in HL-60 cells (blue) or using an isotype control (red).

All lanes : Anti-ACAA1 antibody [2H6BE7] (ab110289) at 1 µg/mlLane 1 : Human liverLane 2 : HepG2Lane 3 : Rat liverLane 4 : Mouse liverLysates/proteins at 10 µg per lane.