Immunocytochemistry image of ab110290 stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (9H10AB4, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
ab110290 immunocaptured from HepG2 cells (lane 1) and Human liver mitochondria (lane 2)
ab110290, at 1 µg/mL, staining ACAT1 in HeLa (blue) or in an isotype control antibody (red) and analyzed by flow cytometry.
ACAT1 immunohistochemistry in human cerebellum visualized with ab110290. ACAT1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of ACAT1 immunoreactivity in the Purkinje cell bodies. The functional significance of this pattern is unknown at present but this antibody offers the opportunity to investigate it in more detail.