Anti-Acetylated Proteins antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab193
• Prices: $394.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP ELISA ChIP
• Antigen: Chemically acetylated human histone H4
• Description: Rabbit Polyclonal
• Conjugate: Unconjugated

Product images

Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 5µl of  ab193 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.     

developed using the ECL techniqueExposure time : 5 minutesImage courtesy of an Abreview submitted by J Stanley.

Product References

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