![Overlay histogram showing SH-SY5Y cells stained with ab118154 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118154, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2009_ab118154-7-ab118154FC.jpg)
Overlay histogram showing SH-SY5Y cells stained with ab118154 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118154, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-ACSBG1 antibody [2B6] (ab118154) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ACSBG1 cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2010_ACSBG1-Primary-antibodies-ab118154-1.jpg)
All lanes : Anti-ACSBG1 antibody [2B6] (ab118154) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY ACSBG1 cDNALysates/proteins at 5 µg per lane.
Immunohistochemical staining of paraffin-embedded Human colon tissue using ab118154 at a dilution of 1/50.
Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab118154 at a dilution of 1/50.
Immunohistochemical staining of paraffin-embedded Human liver tissue using ab118154 at a dilution of 1/50.
Immunohistochemical staining of paraffin-embedded Human Adenocarcinoma of ovary tissue using ab118154 at a dilution of 1/50.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab118154 at a dilution of 1/50