Anti-ACSL5 antibody
| Name | Anti-ACSL5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57210 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: PQPVLPLLDL NNQSVGIEGG ARKGVSQKNN DLTSCCFSDA KTMYEVFQRG LAVSDNGPCL GYRKPNQPYR WLSYKQVSDR AEYLGSCLLH KGYKSS, corresponding to amino acids 91-187 of Human ACSL5 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | ACSL5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HepG2 cells stained with ab57210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57210, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2056_ab57210-3-ab57210FC.jpg)
Overlay histogram showing HepG2 cells stained with ab57210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57210, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ACSL5 antibody (ab57210) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
ACSL5 antibody (ab57210) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
Product References
Increased long chain acyl-Coa synthetase activity and fatty acid import is linked - Increased long chain acyl-Coa synthetase activity and fatty acid import is linked
Nchoutmboube JA, Viktorova EG, Scott AJ, Ford LA, Pei Z, Watkins PA, Ernst RK, Belov GA. PLoS Pathog. 2013;9(6):e1003401.
Gene expression profiling in sinonasal adenocarcinoma. - Gene expression profiling in sinonasal adenocarcinoma.
Tripodi D, Quemener S, Renaudin K, Ferron C, Malard O, Guisle-Marsollier I, Sebille-Rivain V, Verger C, Geraut C, Gratas-Rabbia-Re C. BMC Med Genomics. 2009 Nov 10;2:65.