Anti-Actin antibody [2Q1055]
| Name | Anti-Actin antibody [2Q1055] |
|---|---|
| Supplier | Abcam |
| Catalog | ab14128 |
| Prices | $392.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 2Q1055 |
| Applications | ICC/IF ICC/IF WB ICC/IF |
| Species Reactivities | Chicken, Yeast, C. elegans, Mammalian, Dictyostelium discoideum, Vertebrates |
| Antigen | Recombinant fragment corresponding to Chicken Actin aa 50-70 (N terminal) |
| Description | Mouse Monoclonal |
| Gene | ACTA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab14128 staining Actin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab14128 at 5μg/ml and ab6046 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Goat anti-Mouse Alexa Fluor 488 secondary (ab150117) at 2 μg/ml (shown in green) and Goat anti-Rabbit Alexa Fluor 594 secondary (ab150084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
![All lanes : Anti-Actin antibody [2Q1055] (ab14128) at 1/1000 dilutionLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : MDA-MB-231 (Breast Carcinoma cell line)Lane 6 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissueLane 7 : Heart (Human) Tissue Lysate - adult normal tissueLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/2140_ab14128-218891-Greyab141281.jpg)
All lanes : Anti-Actin antibody [2Q1055] (ab14128) at 1/1000 dilutionLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : MDA-MB-231 (Breast Carcinoma cell line)Lane 6 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissueLane 7 : Heart (Human) Tissue Lysate - adult normal tissueLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (Alexa Fluor® 790) (ab175783) at 1/10000 dilution
Product References
Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid - Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid
Pougovkina O, te Brinke H, Ofman R, van Cruchten AG, Kulik W, Wanders RJ, Houten SM, de Boer VC. Hum Mol Genet. 2014 Jul 1;23(13):3513-22.
A systems biology-based investigation into the therapeutic effects of Gansui - A systems biology-based investigation into the therapeutic effects of Gansui
Zhang Y, Guo X, Wang D, Li R, Li X, Xu Y, Liu Z, Song Z, Lin Y, Li Z, Lin N. Sci Rep. 2014 Feb 24;4:4154.
Targeting inhibition of fibroblast activation protein-alpha and prolyl - Targeting inhibition of fibroblast activation protein-alpha and prolyl
Christiansen VJ, Jackson KW, Lee KN, Downs TD, McKee PA. Neoplasia. 2013 Apr;15(4):348-58.
Diacylated lipopeptide from Mycoplasma synoviae mediates TLR15 induced innate - Diacylated lipopeptide from Mycoplasma synoviae mediates TLR15 induced innate
Oven I, Resman Rus K, Dusanic D, Bencina D, Keeler CL Jr, Narat M. Vet Res. 2013 Oct 17;44:99.
Reduced coupling of oxidative phosphorylation in vivo precedes electron transport - Reduced coupling of oxidative phosphorylation in vivo precedes electron transport
Siegel MP, Kruse SE, Knowels G, Salmon A, Beyer R, Xie H, Van Remmen H, Smith SR, Marcinek DJ. PLoS One. 2011;6(11):e26963.
A simple yeast-based system for analyzing inhibitor resistance in the human - A simple yeast-based system for analyzing inhibitor resistance in the human
Millson SH, Prodromou C, Piper PW. Biochem Pharmacol. 2010 Jun 1;79(11):1581-8.
Methylation and demethylation activities of a C. elegans MLL-like complex - Methylation and demethylation activities of a C. elegans MLL-like complex
Fisher K, Southall SM, Wilson JR, Poulin GB. Dev Biol. 2010 May 1;341(1):142-53.
Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors. - Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors.
Yoshimura S, Gerondopoulos A, Linford A, Rigden DJ, Barr FA. J Cell Biol. 2010 Oct 18;191(2):367-81.
Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields - Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields
Kyei GB, Dinkins C, Davis AS, Roberts E, Singh SB, Dong C, Wu L, Kominami E, Ueno T, Yamamoto A, Federico M, Panganiban A, Vergne I, Deretic V. J Cell Biol. 2009 Jul 27;186(2):255-68.