Anti-Actin antibody - Loading Control
| Name | Anti-Actin antibody - Loading Control |
|---|---|
| Supplier | Abcam |
| Catalog | ab1801 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Rat, Chicken, Bovine, Human, Xenopus, Drosophila, Zebrafish, Rabbit, Yeast, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Actin |
| Description | Rabbit Polyclonal |
| Gene | ACTA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab1801 staining Actin in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1801, 5μl/ml concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Brain (Rat) Tissue LysateLane 3 : Brain (Mouse) Tissue LysateLane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilutionLane 1 : Whole cell lysates prepared from HUVEC cellsLane 2 : Whole cell lysates prepared from HUVEC cellsLane 3 : Whole cell lysates prepared from HUVEC cellsLane 4 : Whole cell lysates prepared from HUVEC cellsLysates/proteins at 15 µg per lane.SecondaryHRP-conjugated goat polyclonal to rabbit Ig at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
Anti-Actin antibody - Loading Control (ab1801) (Sample: ab73630, 0.1 ug)SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
Product References
Effects of secreted factors in culture medium of annulus fibrosus cells on - Effects of secreted factors in culture medium of annulus fibrosus cells on
Moon HJ, Yurube T, Lozito TP, Pohl P, Hartman RA, Sowa GA, Kang JD, Vo NV. Osteoarthritis Cartilage. 2014 Feb;22(2):344-54.
Mangiferin has an additive effect on the apoptotic properties of hesperidin in - Mangiferin has an additive effect on the apoptotic properties of hesperidin in
Bartoszewski R, Hering A, Marszall M, Stefanowicz Hajduk J, Bartoszewska S, Kapoor N, Kochan K, Ochocka R. PLoS One. 2014 Mar 14;9(3):e92128.
Human amniotic fluid stem cells possess the potential to differentiate into - Human amniotic fluid stem cells possess the potential to differentiate into
Yu X, Wang N, Qiang R, Wan Q, Qin M, Chen S, Wang H. Biol Reprod. 2014 Apr 3;90(4):73.
The PIKE homolog Centaurin gamma regulates developmental timing in Drosophila. - The PIKE homolog Centaurin gamma regulates developmental timing in Drosophila.
Gundner AL, Hahn I, Sendscheid O, Aberle H, Hoch M. PLoS One. 2014 May 20;9(5):e97332.
Disruption of myocardial Gata4 and Tbx5 results in defects in cardiomyocyte - Disruption of myocardial Gata4 and Tbx5 results in defects in cardiomyocyte
Misra C, Chang SW, Basu M, Huang N, Garg V. Hum Mol Genet. 2014 Oct 1;23(19):5025-35.
Nitrosative modifications of the Ca2+ release complex and actin underlie - Nitrosative modifications of the Ca2+ release complex and actin underlie
Yamada T, Fedotovskaya O, Cheng AJ, Cornachione AS, Minozzo FC, Aulin C, Friden C, Turesson C, Andersson DC, Glenmark B, Lundberg IE, Rassier DE, Westerblad H, Lanner JT. Ann Rheum Dis. 2014 May 22. pii: annrheumdis-2013-205007. doi:
Re-engineered p53 activates apoptosis in vivo and causes primary tumor regression - Re-engineered p53 activates apoptosis in vivo and causes primary tumor regression
Okal A, Matissek KJ, Matissek SJ, Price R, Salama ME, Janat-Amsbury MM, Lim CS. Gene Ther. 2014 Oct;21(10):903-12.
Revealing long noncoding RNA architecture and functions using domain-specific - Revealing long noncoding RNA architecture and functions using domain-specific
Quinn JJ, Ilik IA, Qu K, Georgiev P, Chu C, Akhtar A, Chang HY. Nat Biotechnol. 2014 Sep;32(9):933-40.
X-inactivation normalizes O-GlcNAc transferase levels and generates an - X-inactivation normalizes O-GlcNAc transferase levels and generates an
Olivier-Van Stichelen S, Hanover JA. Front Genet. 2014 Aug 4;5:256.
Umbelliferone and daphnetin ameliorate carbon tetrachloride-induced - Umbelliferone and daphnetin ameliorate carbon tetrachloride-induced
Mohamed MR, Emam MA, Hassan NS, Mogadem AI. Environ Toxicol Pharmacol. 2014 Sep;38(2):531-41. doi: