Anti-active Caspase-3 antibody
| Name | Anti-active Caspase-3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2302 |
| Prices | $400.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Quail |
| Antigen | Synthetic peptide corresponding to Human active Caspase-3 |
| Blocking Peptide | Human Caspase-3 peptide |
| Description | Rabbit Polyclonal |
| Gene | CASP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2302 staining active Caspase-3 in human ulcerative colitis, colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed using the HOPE method and permeabilized. Samples were incubated with primary antibody (1/20) for 1 hour at 25°C. A Biotin-SP-conjugated donkey anti-rabbit IgG polyclonal (1/800) was used as the secondary antibody.See Abreview
Lung sections were stained with caspase antibody ab2302. Caspase-3 positive cells are shown in brown (DAB). This picture was kindly supplied as part of a customer review.
Anti-active Caspase-3 antibody (ab2302) at 1 µg/ml + Camptothecin (2 µM) treated Jurkat cells.
ab2302 at a 1/20 dilution staining active caspase-3 from rat pancreas tissue sections by IHC-P. Heat mediated antigen retrieval was used and the tissue was formaldehyde fixed and blocked with 20% serum. The primary antibody was then incubated with the tissue for 30 minutes. A HRP conjugated goat polyclonal was used as the secondary antibody.See Abreview
ab2302 at 1/50 staining mouse embryo (day 16) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed in citric acid. The tissue was then blocked and incubated with the antibody for 1 hour. A biotinylated goat anti-rabbit antibody was used as the secondary. The image shows an E16 mouse body parasaggital sections showinf DRGs in the lumbar/sacral region of the spinal column (there is highest apoptotic index in the organ at this age).See Abreview
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of active Caspase-3 antibody (ab2302) on Quail Tissue sections (Quail E6/7 developing DRGs Saggital section). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins RT. Primary Antibody ab2302 incubated at 1/50 for at RT. Secondary Antibody: Biotin conjugated goat anti rabbit IgG (1/300).
Product References
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