Anti-ADAM10 antibody
| Name | Anti-ADAM10 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab1997 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide, corresponding to amino acids 732-748 of Human ADAM10 |
| Description | Rabbit Polyclonal |
| Gene | ADAM10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab1997 at 2µg/ml staining K562 cells by ICC/IF.
All lanes : Anti-ADAM10 antibody (ab1997) at 1/1666 dilutionLane 2 : ADAM10 siRNA treated human keratinocyte cellsLane 3 : Non-treated human keratinocyte cells.SecondaryHRP conjugated Goat anti-rabbit antibodydeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab1997 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1997, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of ADAM10 in K562 cells using ab1997 at 10 ug/ml.
Lane 1 : Anti-ADAM10 antibody (ab1997) at 1 µg/mlLane 2 : Anti-ADAM10 antibody (ab1997) at 2 µg/mlLane 1 : Jurkat whole cell lysateLane 2 : Jurkat whole cell lysate
All lanes : Anti-ADAM10 antibody (ab1997) at 1/1000 dilutionLane 1 : Human frontal cortex tissue lysate - wholeLane 2 : Human frontal cortex tissue lysate - wholeLysates/proteins at 20 µg per lane.SecondaryIRDye® 800CW-conjugated donkey anti-rabbit IgG polyclonal at 1/18000 dilutionPerformed under reducing conditions.
Product References
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ADAM metalloproteases promote a developmental switch in responsiveness to the - ADAM metalloproteases promote a developmental switch in responsiveness to the
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The transcription factor PAX2 regulates ADAM10 expression in renal cell - The transcription factor PAX2 regulates ADAM10 expression in renal cell
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Junctional adhesion molecule-C is a soluble mediator of angiogenesis. - Junctional adhesion molecule-C is a soluble mediator of angiogenesis.
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SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene - SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene
Donmez G, Wang D, Cohen DE, Guarente L. Cell. 2010 Jul 23;142(2):320-32.
A disintegrin and metalloproteinase-10 (ADAM-10) mediates DN30 antibody-induced - A disintegrin and metalloproteinase-10 (ADAM-10) mediates DN30 antibody-induced
Schelter F, Kobuch J, Moss ML, Becherer JD, Comoglio PM, Boccaccio C, Kruger A. J Biol Chem. 2010 Aug 20;285(34):26335-40.
nELAV proteins alteration in Alzheimer's disease brain: a novel putative target - nELAV proteins alteration in Alzheimer's disease brain: a novel putative target
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