
Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line (from left to right), using Mouse Hmga2 Antibody (N-term)(Cat. #AP21032a). AP21032a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Western blot analysis of lysate from NCCIT cell line, using Mouse Hmga2 Antibody (N-term)(Cat. #AP21032a). AP21032a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

All lanes : Anti-Hmga2 Antibody (N-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: 293T whole cell lysates Lane 3: MDA-MB-231 whole cell lysates Lane 4: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.