Anti-ADAM17 antibody

• Supplier: Abcam
• Catalog: ab2051
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide, corresponding to amino acids 807-823 of Human ADAM17
• Description: Rabbit Polyclonal
• Gene: ADAM17
• Conjugate: Unconjugated

Product images

All lanes : Anti-ADAM17 antibody (ab2051) at 1/500 dilutionLane 1 : HeLa whole cell lysate with absence of blocking peptideLane 2 : Jurkat whole cell lysate with absence of blocking peptideLane 3 : HeLa whole cell lysate with ADAM17 peptide (ab7881)Lane 4 : Jurkat whole cell lysate with ADAM17 peptide (ab7881)

Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/mlLane 2 : Unrelated antibody of the same subclass as ab2051.Lane 1 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cellsLane 2 : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cellsSecondaryHRP conjugated goat polyclonal antibodydeveloped using the ECL techniquePerformed under reducing conditions.

ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF

All lanes : Anti-ADAM17 antibody (ab2051) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : U937 cell lysateLane 3 : HT1080 cell lysateLane 4 : ES cell lysateLane 5 : L929 cell lysateLane 6 : CHO cell lysateLane 7 : 293T cell lysateLane 8 : ADAM17-overexpressing 293T cell lysateLysates/proteins at 50 µg per lane.SecondaryHRP-conjugated Goat anti-Rabbit polyclonal antibody at 1/4000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

IHC image of ADAM17 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2051, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunofluorescence of TACE in HeLa cells using ab2051 at 10 ug/ml.

Product References

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