Anti-ADAM17 antibody - Cytoplasmic domain
| Name | Anti-ADAM17 antibody - Cytoplasmic domain |
|---|---|
| Supplier | Abcam |
| Catalog | ab39162 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Rat, Human, Pig |
| Antigen | Synthetic peptide based on the cytoplasmic domain of full length human ADAM17 |
| Description | Rabbit Polyclonal |
| Gene | ADAM17 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab39162 was used to detect mouse ADAM17 transiently overexpressed in Hela cells. Ab39162 was incubated with the cells at 10 µg/ml for 1 hour at 22°C. For further details please refer to the Abreview.
Ab39162 staining human normal colon. Staining is localised to membrane.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Dual cleavage of neuregulin 1 type III by BACE1 and ADAM17 liberates its EGF-like - Dual cleavage of neuregulin 1 type III by BACE1 and ADAM17 liberates its EGF-like
Fleck D, van Bebber F, Colombo A, Galante C, Schwenk BM, Rabe L, Hampel H, Novak B, Kremmer E, Tahirovic S, Edbauer D, Lichtenthaler SF, Schmid B, Willem M, Haass C. J Neurosci. 2013 May 1;33(18):7856-69.
Plasmalogens inhibit APP processing by directly affecting gamma-secretase - Plasmalogens inhibit APP processing by directly affecting gamma-secretase
Rothhaar TL, Grosgen S, Haupenthal VJ, Burg VK, Hundsdorfer B, Mett J, Riemenschneider M, Grimm HS, Hartmann T, Grimm MO. ScientificWorldJournal. 2012;2012:141240.
Differential neuregulin 1 cleavage in the prefrontal cortex and hippocampus in - Differential neuregulin 1 cleavage in the prefrontal cortex and hippocampus in
Marballi K, Cruz D, Thompson P, Walss-Bass C. PLoS One. 2012;7(5):e36431.
Docosahexaenoic acid reduces amyloid beta production via multiple pleiotropic - Docosahexaenoic acid reduces amyloid beta production via multiple pleiotropic
Grimm MO, Kuchenbecker J, Grosgen S, Burg VK, Hundsdorfer B, Rothhaar TL, Friess P, de Wilde MC, Broersen LM, Penke B, Peter M, Vigh L, Grimm HS, Hartmann T. J Biol Chem. 2011 Apr 22;286(16):14028-39.
Docosahexaenoic acid reduces amyloid beta production via multiple pleiotropic - Docosahexaenoic acid reduces amyloid beta production via multiple pleiotropic
Grimm MO, Kuchenbecker J, Grosgen S, Burg VK, Hundsdorfer B, Rothhaar TL, Friess P, de Wilde MC, Broersen LM, Penke B, Peter M, Vigh L, Grimm HS, Hartmann T. J Biol Chem. 2011 Apr 22;286(16):14028-39.