Anti-ADAMTS1 antibody - Aminoterminal end

• Supplier: Abcam
• Catalog: ab28284
• Prices: $390.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P B/N WB ICC/IF ICC/IF
• Species Reactivities: Human, Monkey
• Antigen: Synthetic peptide based on the aminoterminal end of furin cleaved human ADAMTS-1
• Description: Rabbit Polyclonal
• Gene: ADAMTS1
• Conjugate: Unconjugated

Product images

Anti-ADAMTS1 antibody - Aminoterminal end (ab28284) at 1/2500 dilution + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

ICC/IF image of ab28284 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab28284 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

ADAMTS4 and ADAMTS5 knockout mice are protected from versican but not aggrecan or - ADAMTS4 and ADAMTS5 knockout mice are protected from versican but not aggrecan or

Demircan K, Topcu V, Takigawa T, Akyol S, Yonezawa T, Ozturk G, Ugurcu V, Hasgul R, Yigitoglu MR, Akyol O, McCulloch DR, Hirohata S. Biomed Res Int. 2014;2014:693746.

Systematic analysis of protease gene expression in the rhesus macaque ovulatory - Systematic analysis of protease gene expression in the rhesus macaque ovulatory

Peluffo MC, Murphy MJ, Baughman ST, Stouffer RL, Hennebold JD. Endocrinology. 2011 Oct;152(10):3963-74.

PGF2alpha-F-prostanoid receptor signalling via ADAMTS1 modulates epithelial cell - PGF2alpha-F-prostanoid receptor signalling via ADAMTS1 modulates epithelial cell

Keightley MC, Sales KJ, Jabbour HN. BMC Cancer. 2010 Sep 14;10:488.