Anti-ADH1B antibody (ab175515) at 1/1000 dilution + HepG2 cell line lysate at 35 µgSecondaryAnti-rabbit HRP conjugated antibody at 1/10000 dilutiondeveloped using the ECL technique
Immunofluorescence analysis of HepG2 cells labeling ADH1B with ab175515 at a 1/10 dilution, followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemical analysis of formalin fixed, paraffin-embedded Human hepatocarcinoma tissue labeling ADH1B with ab175515 at a 1/50 dilution, followed by DAB staining.
Flow cytometry analysis of HepG2 cells labeling ADH1B with ab175515 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.