AEBP2 (D7C6X) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 14129
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D7C6X
• Applications: WB IP ChIP
• Species Reactivities: Human, Mouse, Rat, Monkey, Hamster, Chicken, Bovine
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu345 of human AEBP2 protein.
• Description: Rabbit Monoclonal
• Gene: AEBP2

Product images

Western blot analysis of extracts from various cell lines using AEBP2 (D7C6X) Rabbit mAb.

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® AEBP2 siRNA I #14711 (+), or SignalSilence ® AEBP2 siRNA II #14713 (+), using AEBP2 (D7C6X) Rabbit mAb (upper) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower). The AEBP2 (D7C6X) Rabbit mAb confirms silencing of AEBP2 expression, while the α-Actinin (D6F6) XP ® Rabbit mAb is used as a loading control.

Immunoprecipitation of AEBP2 from NCCIT cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or AEBP2 (D7C6X) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AEBP2 (D7C6X) Rabbit mAb.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 NCCIT cells and either 20 µl of AEBP2 (D7C6X) Rabbit mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human HoxA1 Intron 1 Primers #7707, SimpleChIP ® Human HoxA2 Promoter Primers #5517, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.