Anti-Aggrecan antibody [6-B-4]
| Name | Anti-Aggrecan antibody [6-B-4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3778 |
| Prices | $400.00 |
| Sizes | 1 ml |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 6-B-4 |
| Applications | ELISA IHC-P IHC-F WB ICC/IF ICC/IF |
| Species Reactivities | Bovine, Human |
| Antigen | Synthetic peptide: EPEEPFTFAPEI , corresponding to amino acids 413-424 of Human Aggrecan |
| Description | Mouse Monoclonal |
| Gene | ACAN |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab3778 at 1/100 staining human articular cartilage by IHC-P. The tissue was formaldehyde fixed and blocked with a serum free protein block. An enzymatic antigen retrieval step was performed and the tissue was then incubated with the antibody for 1 hour. An HRP conjugated goat polyclonal antibody was used as the secondary.See Abreview
ab3778 used at a 1/50 dilution staining Aggrecan in human umbilical cord matrix mesenchymal stromal cells by Immunocytochemistry/ Immunofluorescence.Frozen sections of the 3D constructs (10µm) (n=2) were rehydrated with PBS for 5 minutes, and endogenous peroxidase activity was inhibited using 1% hydrogen peroxide in methanol for 30 minutes. The sections were then blocked in 3% horse serum for 20 minutes and incubated with a primary antibody, ab3778, for 1 hour at a 1/50 dilution. After primary antibody incubation the sections were incubated with a streptavidin linked horse anti-mouse IgG secondary antibody for 30 minutes. After secondary antibody incubation, the sections were incubated with avidin-biotinylated enzyme complex for 30 minutes, and then VIP substrate (purple color) was applied on sections for 4 minutes.The image shown is at 4 weeks, 25M.
Immunofluorescence analysis of Human articular chondrocytes, staining Aggrecan with ab3778. Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour. Cells were incubated with primary antibody overnight at 4°C. An AlexaFluor®488-conjugated rabbit anti-mouse IgG was used as the secondary antibody.
Product References
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Human articular chondrocytes express ChemR23 and chemerin; ChemR23 promotes - Human articular chondrocytes express ChemR23 and chemerin; ChemR23 promotes
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