Anti-Ago2 / eIF2C2 antibody [EPR10411]

• Supplier: Abcam
• Catalog: ab186733
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR10411
• Applications: ICC/IF ICC/IF FC IP WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ago2/ eIF2C2 aa 350-450
• Description: Rabbit Monoclonal
• Gene: AGO2
• Conjugate: Unconjugated

Product images

All lanes : Anti-Ago2 / eIF2C2 antibody [EPR10411] (ab186733) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

All lanes : Anti-Ago2 / eIF2C2 antibody [EPR10411] (ab186733) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Ago2 / eIF2C2 with ab186733 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Ago2 / eIF2C2 with ab186733 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling Ago2 / eIF2C2 with ab186733 at 1/60 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot analysis of Ago2 / eIF2C2 in MCF7 cell lysate immunoprecipitated with ab186733 at 1/50 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.